We offer our experience in assay development for detailed mechanistic studies of a target protein, bioanalytical measurements and high-throughput screening. Since we thoroughly characterize the materials for which an assay is developed, our assays have maximized signal windows, low variability and are economical. These assays can have diverse applications such as analysis of enzyme activities, small molecule binding to proteins, protein-protein interactions and nucleic acid-protein interactions. In addition we offer our services for outsourcing your assays or mechanistic studies.

Functional modes

1. protein-protein, protein-nucleic acid, protein-small molecule or peptide binding interactions
2. receptor functional assays
3. enzymatic activity
4. inhibition

Assay formats

1. 96-well plate
2. single sample (for UV absorbance)
3. HTS-compatible

Preferred detection methods

1. fluorescence intensity
2. fluorescence polarization
3. fluorescence energy resonance transfer (FRET)
4. luminescence
5. light absorbance

Assay targets

1. enzymes
2. antibodies
3. receptors
4. inhibitors
5. peptides
6. small molecules

Assay Deliverables

1. binding site characterization (B_max and K_d)
2. enzyme steady state kinetic parameters determination (K_m and V_max)
3. inhibitor characterization
1. single point inhibition for multiple inhibitors at a specified concentration
2. IC₅₀ and K_i determination
3. analysis of tight binding inhibitor kinetics
4. concentration determination for proteins, peptides and small molecules

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