Protein Characterization

Analytical characterization ensures the identity, purity, structural and conformational integrity and activity of a protein. We perform a number of routine protein analyses throughout all project stages. If needed, we can submit your samples for additional methods of characterization. We also offer specialized protein characterization services for purified proteins.

Analysis of raw, intermediate and final protein products

1. electrophoresis (SDS-PAGE, native-PAGE, IEF-PAGE, urea-PAGE)
2. Western/dot blot
3. enzyme activity assay by light absorbance or fluorescence
4. ELISA (direct or sandwich)
5. protein assay (A280, BCA or equivalent)
6. antibody isotyping
7. endotoxin measurement (LAL assay)
8. contaminating DNA assay
9. UV-Vis absorption spectrum

Preparation and submission of protein samples for

1. amino acid analysis
2. N-terminus analysis
3. protein identification and analysis by mass-spectrometry
4. extinction coefficient determination

Protein characterization

1. analytical size exclusion chromatography (native MW estimate, aggregation analysis)
2. analytical ion-exchange chromatography (oxidation)
3. purity analysis by densitometry
4. UV-Vis light absorption spectroscopy
5. analysis of oxidation, degradation and aggregation products
6. protein deglycosylation analysis
7. protein/protein interactions by co-immunoprecipitation, spectroscopy, chromatography or BIACORE
8. binding site characterization (n, B_max, K_d, Hill coefficient)
9. enzyme steady state kinetics (K_m and V_max)
10. inhibitor characterization - type of inhibition, IC₅₀ and K_i determination

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