ARVYS Proteins Inc.

Protein Expression Services

Our Protein Expression Services incorporate the latest developments in bacterial, baculovirus and mammalian expression technologies to assure that your recombinant protein production will be efficient and economical. We offer a number of Protein Expression Services designed to meet specific goals, ranging from expression of tagged proteins for research to production of biopharmaceuticals for clinical use. Your custom protein expression will be optimized by engineering an expression construct with a codon-optimized DNA insert, a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host. High level of protein expression also depends on stability, solubility, and folding pathway of the protein product. Accordingly, we will optimize these protein expression parameters when such optimization is necessary.

Request a Quote.

BACTERIAL PROTEIN EXPRESSION

  1. Codon optimization for bacterial expression and gene synthesis of DNA insert
  2. Generation of bacterial expression construct(s) in a selected or client-specified vector
  3. Transformation into an appropriate host and preparation of glycerol stocks
  4. Screening studies for the best-expressing protein variant
  5. Optimization of growth conditions (host, induction, media, temperature, additives) to drive either soluble or inclusion bodies expression
  6. Soluble vs. inclusion bodies expression assessment by SDS-PAGE/Coomassie or Western blot
  7. Bacterial paste scale up production (read more Fermentation and Cell Culture Services)
  8. Inclusion bodies wash and recovery
  9. Recombinant Protein purification from soluble lysate fraction (read more Protein Purification Services)
  10. Recombinant Protein purification from inclusion bodies by refolding (read more Protein Purification Services)

BACULOVIRUS PROTEIN EXPRESSION

  1. Codon optimization for insect cell expression and gene synthesis of DNA insert
  2. Subcloning of a DNA insert into a selected or client-specified baculovirus expression vector
  3. Virus generation, amplification and cloning by limited dilution or plaque purification
  4. Generation of high-titer virus stocks
  5. SDS-PAGE, Western, ELISA or functional expression analysis of cell lysates and conditioned media for intracellular and secreted protein products
  6. Secreted or intracellular expression in SF9, SF21 and High Five insect cells
  7. Expression optimization studies: MOI, time course, growth media
  8. Large-scale insect cell culture for conditioned media or cell pellet production
    (read more Fermentation and Cell Culture Services)
  9. Recombinant protein purification from conditioned media or cell pellet (read more Protein Purification Services)

MAMMALIAN PROTEIN EXPRESSION

  1. Codon optimization for mammalian cell expression and gene synthesis of DNA insert
  2. Generation of mammalian protein expression construct(s) in a selected or client-specified vector
  3. Endotoxin-free plasmid DNA preparation for transient or stable transfections
  4. Small-scale trial to assess recombinant protein expression and/or to optimize transient transfection conditions
  5. Various transient transfection reagent options
  6. SDS-PAGE, Western, ELISA or functional expression analysis of conditioned media and/or cell lysates
  7. Secreted or intracellular expression in CHO, HEK293, HEK293E or client-specified cells
  8. Adherent or suspension cell growth options
  9. Large-scale mammalian cell culture for conditioned media or cell pellet production
    (read more Fermentation and Cell Culture Services)
  10. Recombinant protein purification from conditioned media or cell pellet (read more Protein Purification Services)

AUXILLARY Protein expression SERVICES include

  1. Long-term storage for DNA, glycerol and virus stocks
  2. Master and working cell banking

Site Map | Disclaimer | Privacy Policy | Blog

Protein Expression Services
var sc_project=7538783; var sc_invisible=1; var sc_security="ddea32e5";