Protein Expression Services

High level of protein expression is critical for efficient and economical purification of active recombinant proteins. We offer a number of protein expression services designed to meet specific goals, ranging from expression of tagged proteins as antigens for antibody generation to production of biopharmaceuticals for clinical use. Our experts can optimize expression by engineering an expression vector with a strong promoter, an efficient ribosome binding site, a high copy number and combining it with an appropriate host. Expression also depends on the gene sequence and on stability, solubility, and folding pathway of the protein product. Accordingly, we will optimize these protein expression parameters when such optimization is necessary.

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BACTERIAL PROTEIN EXPRESSION SERVICES

1. Generation of bacterial expression construct(s) in a selected or customer-specified vector
2. Transformation into an appropriate host and preparation of glycerol stocks
3. Screening studies for the best-expressing protein variant
4. Optimization of expression conditions: host, induction, media, temperature, additives
5. Soluble vs. inclusion bodies expression assessment by SDS-PAGE/Coomassie or Western blot
6. Bacterial paste scale up production (Read More Raw Material Production)
7. Inclusion bodies wash and recovery
8. Recombinant Protein purification from soluble lysate fraction (Read More Protein Purification)
9. Recombinant Protein purification from inclusion bodies by refolding (Read More Protein Purification)

BACULOVIRUS PROTEIN EXPRESSION SERVICES

1. Subcloning of cDNA into a selected or customer-specified baculovirus transfer vector
2. Virus amplification and cloning by limited dilution or plaque purification
3. Generation of high-titer virus stocks
4. SDS-PAGE, Western, ELISA or functional expression analysis of cell lysates and conditioned media for intracellular and secreted protein products
5. Expression in SF9, SF21 and High Five insect cells
6. Expression optimization studies: MOI, time course, growth media
7. Large-scale insect cell fermentation for conditioned media or cell pellet production
   (Read More Raw Material Production)
8. Recombinant protein purification from conditioned media or cell pellet (Read More Protein Purification)

MAMMALIAN PROTEIN EXPRESSION SERVICES

1. Generation of mammalian protein expression construct(s) in a selected or customer-specified vector
2. Endotoxin-free DNA preparation for transient or stable transfections
3. Small-scale trial to assess recombinant protein expression and/or to optimize transient transfection conditions
4. Various transient transfection reagent options
5. SDS-PAGE, Western, ELISA or functional expression analysis of conditioned media and/or cell lysates
6. Secreted or intracellular expression in CHO, HEK293, HEK293E or customer-specified cells
7. Adherent or suspension cell growth options
8. Large-scale mammalian cell culture for conditioned media or cell pellet production
   (Read More Raw Material Production)
9. Recombinant protein purification from conditioned media or cell pellet (Read More Protein Purification)

AUXILLARY SERVICES

1. Long-term storage for DNA, glycerol and virus stocks
2. Master and working cell banking

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