Raw Materials Production

Raw materials determine the purification strategy or even the success of purification. Protein extraction/recovery could be a very important purification step in itself. Time and effort invested at this stage often result in substantially shorter purification protocols and higher yields of active protein. Our experience with various methods of protein extraction, fractionation and enrichment for both recombinant and natural sources enables us to design the most optimal production process for your starting materials.

Production of recombinant proteins

1. conditioned media up to 200L
2. mammalian cell pellet
3. bacterial paste from up to 50L of culture

Lysis of tissues or cell pellets

1. sonication
2. homogenization
3. detergent solubilization
4. hypotonic solution treatment
5. combination of the above

Inclusion bodies refolding screen

Raw materials enrichment by

1. ammonium sulfate precipitation
2. sucrose gradient centrifugation
3. differential detergent extraction
4. partition into Triton X-114
5. treatment for membrane preparations
6. inclusion body isolation, wash, solubilization and refolding

Concentration of raw materials by

1. centrifugation
2. tangential flow filtration (TFF)

Quantitative analysis of the target protein expression

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