What We Offer

Why do you need expert help to purify and characterize proteins?

1. Your protein which appears as a single protein band on SDS-PAGE could be heterogeneous due to:
1. Aggregated, modified or partially degraded forms of the target protein
2. Other proteins with the same rate of migration on SDS-PAGE
3. Products from faster growing cells which may come from incomplete clonal selection or clone modification
4. Presence of highly active impurities which are not easily visualized, such as endotoxin
2. Heterogeneity in preparations results in the following problems:
1. Presence of even small amounts of denatured or misfolded proteins may trigger further protein denaturation leading to instability during manipulations and storage
2. Small impurities may contribute disproportionately to the product concentration measurements resulting in erroneous data interpretation.
3. In in vivo studies, denatured protein, contaminants and endotoxin may induce unwanted biological responses
4. In screening experiments, denatured protein may bind inhibitors and significantly affect K_i and IC₅₀ values
3. Your protein preparation may be fully or partially inactive due to:
1. Failure to form a native conformation during production or renaturation procedures
2. Denaturation, degradation or modification
3. Alteration by protein tagging or labeling
4. Shielding of an active site by contaminating ligands
4. Loss or alteration of functional activity results in the following problems:
1. Ligand binding parameters are changed
2. Larger amounts of protein have to be used
3. Result consistency may be affected
4. Increased contribution from background activity reduces assay window
5. Biological responses are too low to be measured
5. Your protein preparation may generate inconsistent and unreliable data.

Get Arvys expertise

1. We integrate our expertise in genetic engineering, protein expression, protein purification, protein characterization and assay development for each project to ensure project's success.
2. We monitor activity and stability of proteins through every step of purification procedures thus minimizing presence of aggregated or denatured target protein in your preparations.
3. Our instrumentation and experience allow us to develop a variety of biochemical and biophysical assays and use them for protein, peptide, and small molecule characterization.
4. We reduce your cost by maximizing the activity and stability of your protein product.
5. We deliver endotoxin-free active proteins for your in vivo studies.

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