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Endotoxin Removal: General Information

Published on August 20, 2012 by in Home

Endotoxins are toxic protoplasms that are released when bacteria grow, die, and disintegrate. These endotoxins are lipopolysaccharides, which reside in the outer membrane of gram-negative bacteria. The gram-negative cell wall is composed of a thin, inner layer of peptidoglycan and an outer membrane of phospholipids, lipopolysaccharides, and lipoprotein.   PROPERTY              ENDOTOXIN Chemical nature Lipopolysaccharide Relationship

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Endotoxin Removal from Biological Solutions

Published on August 16, 2012 by in Home

Endotoxin Removal from Biological Solutions Summary of Commercial Kits and Services   Each removal technique addresses a different problem regarding endotoxins. The most prominent methods that companies have been using are based on separation by size, which uses filtration; separation by charge, which uses ion-exchange resins, separation by phase, which uses a non-ionic detergent Triton

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Production of Recombinant Proteins in a Soluble Form in E.coli

Published on August 16, 2012 by in Home

Proteins are an essential part of nutrition, medicine, and biochemistry. Modern technology has allowed producing recombinant proteins in large quantities for research and therapeutic use. One of the most common expression systems for protein production is E. coli. However, when heterologous (non-bacterial) proteins are expressed in bacteria, often a produced protein is found in “inclusion

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