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Endotoxin Removal by Triton X-114 partitioning

Published on September 11, 2012 by in Home

A variety of methods have been developed in order to remove endotoxins from protein solutions, with one of the more popular being separation by phase using non-ionic detergent Triton X-114. Triton X-114 detergent is homogeneous at cold temperatures, but once above a critical temperature of 22°C, its solution separates into an aqueous phase, where hydrophilic

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Endotoxin Removal: General Information

Published on August 20, 2012 by in Home

Endotoxins are toxic protoplasms that are released when bacteria grow, die, and disintegrate. These endotoxins are lipopolysaccharides, which reside in the outer membrane of gram-negative bacteria. The gram-negative cell wall is composed of a thin, inner layer of peptidoglycan and an outer membrane of phospholipids, lipopolysaccharides, and lipoprotein.   PROPERTY              ENDOTOXIN Chemical nature Lipopolysaccharide Relationship

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Endotoxin Removal from Biological Solutions

Published on August 16, 2012 by in Home

Endotoxin Removal from Biological Solutions Summary of Commercial Kits and Services   Each removal technique addresses a different problem regarding endotoxins. The most prominent methods that companies have been using are based on separation by size, which uses filtration; separation by charge, which uses ion-exchange resins, separation by phase, which uses a non-ionic detergent Triton

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Production of Recombinant Proteins in a Soluble Form in E.coli

Published on August 16, 2012 by in Home

Proteins are an essential part of nutrition, medicine, and biochemistry. Modern technology has allowed producing recombinant proteins in large quantities for research and therapeutic use. One of the most common expression systems for protein production is E. coli. However, when heterologous (non-bacterial) proteins are expressed in bacteria, often a produced protein is found in “inclusion

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ARVYS Proteins inc.

Published on May 8, 2012 by in Home

ARVYS Proteins Inc. is a contract research organization that specializes in custom protein services. Arvys offers its protein biochemistry expertise to the life science, biotechnology and pharmaceutical communities. We help customers from any research sector at any stage of their protein research and development project, big or small. Our goal is to be your preferred outsourcing

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