Services

Antibody Development & Production

In the last 30 years, Antibodies had become very important reagents in research, diagnostics, and medicine. This class of molecules are heavily used now in many fields of biomedical research such as immunoassays (Western blots, ELISA, etc.), protein purification, protein identification and protein-protein interaction studies. Many commercial diagnostic kits have antibodies as their critical components. Currently, there are 75 Monoclonal Antibodies or their derivatives approved by the FDA and many more are being tested in clinical studies. Each antibody project requires a customized approach gathering all facts about an antigen and defining the properties of the final antibody. We have a long history in developing and producing antibodies for our clients. We will plan your Antibody project thoroughly to achieve desired outcomes.

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PURIFICATION OF ANTIBODIES FROM BIOLOGICAL SOURCES

  • all antibody isotypes from any species
  • endotoxin removal from antibody solutions
  • antibody conjugation to fluorescent dyes or enzymes
  • antibody characterization by ELISA and other assays

PRODUCTION OF RECOMBINANT ANTIBODIES IN MAMMALIAN EXPRESSION SYSTEMS BY TRANSIENT OR STABLE TRANSFECTION


PRODUCTION OF MONOCLONAL ANTIBODIES FROM HYBRIDOMA CELL LINES


PRODUCTION OF POLYCLONAL ANTIBODIES

  • design of an antigen with immunogenic properties
  • antigen generation, production and purification
  • analysis of bleeds for specificity by ELISA and/or Western blot
  • purification of antibodies from blood plasma

PRODUCTION OF SINGLE CHAIN ANTIBODIES (scFv) and Fab FRAGMENTS IN E.COLI

  • design of an expression construct
  • expression in E.coli and its optimization
  • antibody purification

 GENERATION OF Fab AND (Fab)2 FRAGMENTS FROM FULL LENGTH ANTIBODIES

  • optimization of enzymatic digestion reaction
  • removal of heavy chain fragments
  • Fab and (Fab)2 purification

PHAGE DISPLAY FOR GENERATION OF MONOCLONAL ANTIBODIES

  • generation of antibody phage display library from infected/diseased patients or antigen-immunized animals
  • phage display panning and screening
  • sequence analysis
  • generation of scFv, Fabs or whole immunoglobulins in various expression

 CHARACTERIZATION OF ANTIBODIES FOR SPECIFICITY AND SELECTIVITY