Protein Purification Services

Our skills in protein purification were gained over years of work with protein pharmaceuticals, drug targets, biological system components and biochemical reagents. Each protein has its own physico-chemical and biological properties that require tailored procedures for extraction, purification and characterization. Our purification strategy aims to achieve a homogeneous active protein preparation in two to three purification steps. This goal is reached by a thorough selection and optimization of the capture step, incorporation of a gel filtration step to remove aggregates, degradation products and other contaminants, selection of buffer conditions that stabilize biological activity and prevent product degradation. Although most proteins require individual approach, we are confident that we can handle your protein. We will purify it cost-efficiently, characterize it according to your specifications and deliver it to you in an active and application-compatible form.

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Protein Purification features

1. common protein purification scales range from 0.001g to 5g
2. proteins are purified according to customer-specified purity
3. Protein purification methods from
1. customer-supplied protocols
2. published protocols
3. improved protocols from customer-supplied or published protocols
4. de novo protocols tailored to customer's requirements
4. any protein purification mode can be used
1. ion-exchange
2. gel filtration
3. affinity (broad-spectrum)
4. hydrophobic interaction
5. refolding from inclusion bodies
6. Protein purification method development for transfer to a GMP facility
7. dedicated columns are used in each project
8. efficiency is provided by the automation and precision of AKTA systems from Amersham BioSciences (currently GE)

Analysis of protein purification steps and of final products

1. SDS-PAGE and/or dot/Western blotting are routinely used for fraction analysis
2. final protein purity is determined by densitometry from Coomassie-stained gels
3. final products are supplied with a certificate of analysis, purification report and, if applicable, a batch record

Final Product

1. certificate of analysis is tailored to customer specification (see our Protein Characterizationpage).
2. final products are dispensed into customer-specified aliquot sizes

Auxiliary services include

1. development of affinity media
2. refolding
3. preformulation screening
4. endotoxin removal
5. tag removal
6. conjugation to biotin or fluorescent dye

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